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Genetic characterisation of experimental material, gene expression analyses and DNA marker development
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Populations of European beech
and Norway spruce, respectively, are studied under greenhouse
and field conditions in order to genetically characterize the
physiological disposition of individuals in response to various
environmental conditions, to pathogen infections and to yield
related parameters. In order to develop genetic markers for
P. citricola tolerance and susceptibility in beech we
use newly developed DNA-AFLP and cDNA-AFLP systems. For the
ananlysis of protein changes a method to isolate proteins for
different tissues (leaves, stem, roots of trees) and fungi (Mycorrhiza,
Phytophthora) is being developed and two-dimensional
electrophoresis will be established for these samples. In infection
studies with Phytophthora the up- and down-regulated
proteins of beech will be characterized. In these experiments
the local as well as the systemic reaction is investigated.
Furthermore, a subtractive cDNA library from P. citricola-infected
roots that was established in co-operation with vertis Biotechnology
is analyzed, and the results are compared to the Phytophthora-induced
gene expression in potato (A10). Together with the data on proteome
changes the sequence data and the subsequently performed expression
analysis (in co-operation with A1) will give insights into regulated
metabolic pathways and the resources invested in pathogen defense.
The application of the marker developed for tolerance/susceptibility
will than enable us to judge the influence of these changes
on the success of the defense reaction. In Kranzberg forest
studies are carried out on the fructification of beech and spruce
inside and outside the ozone fumigation which includes controlled
crossings.
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Picea
Fagus
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