Work of A7 within the SFB 607:
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Within the framework of SFB 607 the project A7 focuses on the central hypothesis "Growth and Parasite Defense". In phase I it could be shown that with an elevated CO2 concentration leaf resistance of suspectible potato cultivars towards Phytophthora infestans decreased. In the second phase of the SFB investigations will concentrate on potato only, since it exhibited a substantial decrease in susceptibility against late blight under elevated CO2- concentrations. It is planned to verify the results of the studies conducted in climate chambers with greenhouse experiments and in the field. The effect of different CO2- and N- treatments on biomass production and on susceptibility of potato plants will be evaluated with conventional methods of phytopathology as well as with methods of biochemistry and molecular biology.
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Experimental design:
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I. General mechanisms of leaf resistance
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CO2 and N treatments:
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CO2 ambient/60 kg/ha *
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Location:
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greenhouse
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Cultivars:
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Bettina (tolerant)
Kuras (tolerant)
Indira (susceptible)
Bintje (susceptible)
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Pathogen:
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Phytophthora infestans
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II. CO2 induced mechanisms of resistance
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CO2 and N treatments:
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400 ppm/60 kg/ha
400 ppm/160 kg/ha
700 ppm/60 kg/ha
700 ppm/160 kg/ha
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Location:
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greenhouse
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Cultivars:
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Bettina (tolerant)
Indira (susceptible)
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Pathogen:
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Phytophthora infestans
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* All new (phase II) parameters and variants are marked with red color
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III. N influence on CO2 induced resistance in infected potato plants
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CO2 and N treatments:
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400 ppm/60 kg/ha *
400 ppm/160 kg/ha
700 ppm/60 kg/ha
700 ppm/160 kg/ha
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Location:
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greenhouse
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Cultivars:
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Bettina (tolerant)
Kuras (tolerant)
Indira (susceptible)
Bintje (susceptible)
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Pathogen:
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Alternaria solani
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IV. N influence on field resistance
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CO2 and N treatments:
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CO2 ambient/60kg/ha
CO2 ambient/160kg/ha
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Location:
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field
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Cultivars:
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Bettina (N tolerant)
Indira (N susceptible)
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Pathogen:
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Phytophthora infestans
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* All new (phase II) parameters and variants are marked with red color
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Analyses:
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| a) Parameters of Growth: |
b) Parameters of Resistance: |
| Photosynthesis |
Infected leaf area |
| Transpiration |
Salicylate |
| Biomass |
AP24 |
| C/N - Analytics |
Oxilipin |
| Starch |
oxidative burst |
| Lipids |
Ethylene |
| Sugar |
Phytoalexins |
| Sterols |
PR-Proteins |
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* All new (phase II) parameters and variants are marked with red color
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Aims:
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Elucidation of general mechanisms of resistance in infected potato leaves.
Elucidation of CO2-induced mechanisms of resistance in infected potato plants.
Determination of N-influence on CO2-induced resistance in infected potato plants.
Determination of N-influence combined with elevated CO2-treatments on resistance of potato plants against Alternaria solani.
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